AMB Volume 32, Issue 1, March 2016 / Pages 74-80

Oligosaccharide Production from Lactose and Lactulose by β-Galactosidase from Lactobacillus plantarum S30

Zheleva, P., Vasileva, T., Bivolarski, V., Iliev, I.

A β-Galactosidase preparation from ten strains Lactobacillus plantarum was evaluated as a biocatalyst for the synthesis of oligosaccharides. Among them, the enzymes from L. plantarum S26 and L. plantarum S30 were selected for further studies. The correlation between organic acid production, α-galactosidase, β-galactosidase and lactate dehydrogenase activity and the initial concentration of lactose and lactulose was studied.

It could be suggested that the studied β-galactosidase catalyzes the trans-glycosylation reaction at 20% lactose and 20% lactulose concentrations.

Keywords: lactose, lactulose, prebiotic potential, Lactobacillus, prebiotics, β-galactosidase, α-galactosidase

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