AMB Volume 42, Issue 2, 2026 Pages 361-377 https://doi.org/10.59393/amb26420218
Lipase from Streptomyces caelestis AS12: Production, Optimisation, and APPlication as a Detergent Additive
Aguis H., Halla N., Benreguieg M., Benabbou T.A.
Microbial lipases are efficient and eco-friendly biocatalysts widely exploited in industrial applications. In this study, a lipase from Streptomyces caelestis AS12 (GenBank accession no. PP837824), isolated from arid Algerian soil, was investigated for its suitability as a detergent additive. Screening of 94 actinomycete isolates identified strain AS12 as exhibiting high lipase activity (70 ± 2.6 U/mL). Lipase production was optimized using a classical one-variable-at-a-time approach, and maximum activity was achieved under fermentation conditions of 40°C, pH 7.0, 48 h incubation, and agitation at 140 rpm, with Tween 80 and yeast extract as supplements. Partial purification by ammonium sulfate precipitation (80%) increased the specific activity to 118.43 U/mg, corresponding to a 1.36-fold purification. The enzyme displayed optimal activity at pH 7 and 40°C and remained stable over a pH range of 6–8 and temperatures between 30 and 45°C. Kinetic parameters determined using olive oil as substrate were Km = 6.34 mM and Vmax = 0.21 mM/min. Catalytic activity was positively influenced by Ca²+, Mg²+, and ethanol. The lipase remained stable in commercial detergents and efficiently removed chocolate stains, indicating its potential as a detergent additive.
Keywords: Microbial lipases, biocatalysts, industrial applications, actinomycete, detergent
View Full Article