AMB Volume 40, Issue 1, 2024 Pages 10-16 https://doi.org/10.59393/amb24400102
Human Papillomavirus Genotyping among Women and its Relationship with Cervical Cancer in Diyala Province
Al-awsi D.S., Mohsin I.H., Al-Naddawi A.M., Ibraheem H.K.
The study aimed to identify Human Papillomavirus (HPV) and its genotypes prevalent among Iraqi women. They collected 89 cervical swab samples from diagnosed patients at Baghdad Teaching Hospital's Early Detection Clinic. Using PCR technique on 19 samples, they found HPV16 (57.89%) and HPV6 (10.52%) genotypes, while HPV-11, 18, and 45 were absent. HPV 16 and HPV 6 were common in cervical cancer among Iraqi women. Sequencing revealed nucleic acid variants in HPV-6 (124A>C) and HPV-16 (225G>T) E6 genes, resulting in silent effects on the encoded protein. These changes didn't alter amino acid residues (p.74I= and p.L117=). Phylogenetic analysis showed substantial distances between their samples and other viral types, indicating distinct phylogenetic distances between type-6, type-16, and other out-group sequences.
Keywords: cervical cancer, Human Papillomavirus, genotypes, PCR, phylogenetic tree
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