AMB Volume 40, Issue 3, 2024 Pages 373-382 https://doi.org/10.59393/amb24400312
Statistical Optimization of Lipase Production by Aspergillus oryzae Using Olive Mill Wastewater
El Okki A.A.K-E.H., S Djekrif–Dakhmouche., Bennamoun L., M. El Okki E.H., Labani F.Z.K.
Among different sources of lipases, fungal lipases are hydrolytic enzymes owing much importance in industrial applications. However, the high production cost limits the industrial application of lipases. To address this issue, we have attempted to optimize lipase production by Aspergillus oryzae on olive mill wastewater (OMW) based medium. Media optimization is considered one of the best ways to economically produce a large quantity of lipase. The medium components were optimized by a statistical approach for optimal lipase production. The Plackett–Burman design was applied to identify the significant factors and response surface methodology to optimize medium components. The enzyme activity was highest under the following conditions: 3.49 g.L-1 glucose, 5.58 g.L-1 yeast extract and 0.72 g.L-1 CaCl2. A feasibility analysis of lipase production using the optimized data was performed, and at optimized conditions, lipase activity increased from 110.21 U.mL-1 to 212.53 U.mL-1, or an increase of 1.93 times.
Keywords: Lipase activity, Aspergillus oryzae, olive mill wastewater, Plackett–Burman design, response surface methodology
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