AMB Volume 40, Issue 3, 2024 Pages 365-372 https://doi.org/10.59393/amb24400311
Antifungal Activity of Pseudomonas chlororaphis 1S4
Georgieva G., Badalova M., Feyzula F., Nedeva T.
The potential of Pseudomonas chlororaphis 1S4 strain as an antifungal biocontrol agent against three species of the economically important Fusarium pathogens: Fusarium proliferatum C1, Fusarium oxysporum F6, and Fusarium solani F7 is studied. P. chlororaphis 1S4 24 and 48 h fresh and sterilized broth cultures were used to assess their antifungal properties for 168 hours. P. chlororaphis 1S4 imposed fungal growth inhibition of 64-85% against all fungal species, best presented at a relatively early stage of the fungi development (72 h) and sustained throughout the whole cultivation period. The sterilized broth bacterial cultures maintained about 60% - 100% of the fresh culture potential to retard the fungal growth. P. chlororaphis 1S4 ability to produce exocellular phenazine-1-carboxylic acid and siderophores was determined. Their antifungal effect was checked after a high-temperature treatment. The potent antifungal activity of the P. chlororaphis 1S4 strain is speculated to be related to the biosynthesis of these biologically active substances that serve as effective bio-fungicides. Thus, the P. chlororaphis 1S4 antifungal potential could be utilized in agricultural initiatives focused on biocontrol.
Keywords: rhizosphere, Pseudomonas chlororaphis, Fusarium, phytopathogens, biocontrol
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